Just cook it doesn’t cut it: 196 sick from E. coli O103 linked to ground beef

The U.S Centers for Disease Control (CDC) reports several states, and the U.S. Department of Agriculture’s Food Safety and Inspection Service are investigating a multistate outbreak of Shiga toxin-producing Escherichia coli O103 infections linked to ground beef.

Ill people in this outbreak ate ground beef from many sources. Some ground beef has been recalled, but more product contaminated with E. coli O103 may still be on the market or in freezers.

Restaurants, retailers, and institutions should not sell or serve the following recalled ground beef products because they may be contaminated with E. coli O103 and could make people sick:

Grant Park Packing in Franklin Park, Ill., recalled 53,200 pounds of raw ground beef products on April 24, 2019.

Recalled products were sold in 40-lb. bulk cardboard boxes of “North Star Imports & Sales, LLC. 100% GROUND BEEF BULK 80% LEAN/ 20% FAT” marked “FOR INSTITUTIONAL USE ONLY” with lot code GP.1051.18 and pack dates 10/30/2018, 10/31/2018, and 11/01/2018.

Recalled products are labeled with establishment number “EST. 21781” inside the USDA mark of inspection on the boxes.

K2D Foods, doing business as Colorado Premium Foods, in Carrollton, Ga., recalledexternal icon approximately 113,424 pounds of raw ground beef products on April 23, 2019.

Recalled products were sold in two 24-lb. vacuum-packed packages in cardboard boxes containing raw “GROUND BEEF PUCK” with “Use Thru” dates of 4/14/19, 4/17/19, 4/20/19, 4/23/19, 4/28/19, and 4/30/19.

Recalled products are labeled with establishment number “EST. 51308” inside the USDA mark of inspection.

Investigators continue to trace other sources for ground beef eaten by ill people in this outbreak, and more product contaminated with E. coli O103 may be recalled.

Cook ground beef hamburgers and mixtures such as meatloaf to an internal temperature of 160°F. Use a food thermometer to make sure the meat has reached a safe internal temperature. You can’t tell whether meat is safely cooked by looking at it.

For hamburgers, insert thermometer through the side of the patty until it reaches the middle.

(Piping hot doesn’t cut it, UK).

Just cook it doesn’t cut it: 6-year-old in France dies from E. coli

(Thanks to our French colleague, Albert, who forwarded this)

Matthew, a child “full of life, very intelligent despite his disability ” according to his mother, Angélique Gervraud, died February 22, 2019 at the Children’s Hospital of Bordeaux. He had been sick for more than a month after eating an undercooked burger at the beginning of January 2019 says his mom in a forum posted on his Facebook page.

It’s probably poorly cooked mince that has contaminated Matthew, his mom is sure. “Matthew only ate that,” she explains. Matthew developed haemolytic uremic syndrome (HUS) usually linked to shiga-toxin producing E. coli.

And because food safety is simple – that’s sarcasm, which the French may not get —  the transmission of the disease can be avoided by simple actions, which advises the site Public Health France:

  • Cook meat thoroughly and especially minced meat at over 65 ° C(The Ministry of Health published a note to the attention of the professionals of the collective catering from February 2007, with the appearance of the first cases)
  • Avoid giving raw lai, and cheeses made from raw milk to young children. Prefer baked or pasteurized pressed cheese
  • Always wash your hands before cooking
  • Keep cooked and raw foods separately
  • Consume quickly and well warmed leftover food.
  • Do not give untreated water to children or the elderly.

In 2017, 164 cases of HUS were reported in children under 15 years of age. There are a hundred in France in general every year.

UK disease detectives: Supermarket loyalty cards to trace an E coli outbreak

Kathie Grant and Lisa Byrne write in Public Health Matters that in November 2017, supermarket loyalty cards were used to trace the source of a large E coli outbreak affecting mainly men in England. Dr Lisa Byrne leads Public Health England’s surveillance of two key bacteria that lead to food poisoning – E Coli and Listeria. Dr Kathie Grant heads the PHE Gastrointestinal Bacteria Reference Laboratory. The two work together as part of a larger team dedicated to reducing foodborne illness and below tell us how they put the pieces of this puzzle together to find the source.

If you’ve ever had food poisoning you’ll know that feeling of mentally going through everything you ate recently, trying to pinpoint what it was that might have made you ill. It’s our job to do that at a national scale.  We bring together lots of different pieces of  information from the community and the lab to try to find  the source of a food poisoning outbreak and then, working alongside other government agencies,  ensure that more people don’t get sick.

We study and monitor many different stomach bugs – some of which you may never have heard of! While stomach bugs are a part of life, PHE works with organisations such as the Food Standards Agency and the Animal and Plant Health Agency to try and prevent them.

Every so often we see a spike in the number of cases.  When this happens it is important that we find the link between the cases and the cause of their illness.  To do this we need to identify the exact strain of a bug to understand if people have got ill from the exact same source.

Whole Genome Sequencing (WGS) ‒ a relatively new process for showing us the makeup of a bacterium or virus’s genes ‒ has changed the way we can find the cause of an outbreak and stop more people getting ill. You can learn more about the process and how it works in our explainer blog.

Before WGS it could take weeks to identify bacteria and sometimes the bacteria could be missed. This slowed down any investigations  as we could not be sure that all the case histories we were taking could be linked to an outbreak – there was a lot of ‘noise’ and false lines of enquiry. With WGS, we can rapidly and accurately identify if bacteria of cases are the same strain and rule out people from our investigation who just happened to be ill at the same time, but with a different illness.

It has also expanded what the word ‘outbreak’ means as we can link cases across several years and different countries, meaning we can more accurately piece together a picture of how something in the food supply chain impacts human health.

Scientists working in the Gastrointestinal Bacteria reference laboratory at Public Health England. The team are processing samples from people who have reported gastrointestinal symptoms, to understand the exact cause of their illness.

Identifying the source of an outbreak is a lot like putting together a jigsaw puzzle, combining multiple pieces of evidence to get the full picture. Sometimes, a common source is obvious, such as when a group of people get ill after eating the same meal, at the same restaurant, on the same day. But other times, we need to use an arsenal of investigative tools, as was the case in a recent E coli outbreak.

In November 2017 our surveillance system alerted us to 12 cases of E coli O157 – (a particular form of E coli), over a six week window. E coliO157 is a relatively rare cause of food poisoning, with only about 700 cases a year, but it can cause a very severe illness. Because of this, any case of E coli O157 identified by doctors and laboratories must be reported to Public Health England.  We monitor the number of cases with our surveillance systems to find any patterns.

Very quickly our reference laboratory used WGS which showed that the cases had the identical genetic “fingerprint” and the work began to trace the source of infection. The majority of people who became ill were men, which was unusual as E coli outbreaks are often linked to salad items ‒ traditionally more likely to be eaten by women.

It took a few rounds of interviews – carried out by colleagues in local authorities – to zero in on the potential source of food poisoning, and a picture started to emerge that implicated burgers from a particular retailer.

We asked the supermarket to analyse the loyalty card records of those who had become ill, to help identify the particular burger product the cases had eaten.  As you can imagine, there were many different types of burgers supplied by the supermarket and it’s often difficult for people to remember exactly what they ate.

Working with the Food Standards Agency we were able to identify that all the cases had bought a particular brand of burger, leading to a product recall to ensure others didn’t get sick. The recall involved removing all the suspected batches of burgers from the supermarket shelves. The supermarket also contacted people who had bought the burgers, advising them not to eat them and return them for a refund.

Sometimes, as in this case, we can rapidly find what is making people ill and quickly remove it from sale. It’s an exciting role and we get a real sense of satisfaction out of using our skills to help people in this way. Other times it can be more frustrating – some outbreaks remain unsolved and it’s a real worry that people will get sick because we can’t eliminate a threat from food distribution.

The role really keeps us on our toes. Our surveillance systems mean that we have a good sense of patterns of illness across the year and how we can intervene to stop people getting unwell – but changes to food habits can catch us by surprise. For instance, raw milk has become more popular recently, bringing with it all the disease risks you would expect from a product that has come straight from a cow without any treatment to kill off bacteria!

In another case, eight people in the UK were affected while on holiday in Germany that was related to seeds.  The seeds were decoratively used as a garnish on salads and were difficult for cases to remember eating. Nearly 1,000 people in Germany got ill in that outbreak and one of the approaches by authorities was to use tourist photos of food to try and identify the common item in meals that could be making people sick.

Solving food borne illness outbreaks can be a real challenge, but by using a variety of the different tools available to us we can quickly intervene to stop people getting ill.

Was it the bulgogi burger? HUS outbreak in S. Korea linked to McDonald’s

Bulgogi is literally “fire meat,” a Korean-style grilled or roasted dish made of thin, marinated slices of beef or pork, grilled on a barbecue or on a stove-top griddle. It is also often stir-fried in a pan in home cooking

A Bulgogi burger is the same idea.

Korea Centers for Disease Control and Prevention is conducting an epidemiological investigation into a case of 7 children and 1 adult who showed symptoms of food poisoning with vomiting and diarrhea after eating McDonald’s hamburgers on 25 Aug 2017 at a Jeonju branch in North Jeolla.

Authorities are not ruling out the possibility that the 8 individuals contracted hemolytic-uremic syndrome (HUS), widely known as “hamburger disease,” a bacterial infection that can leave the renal system severely damaged.

On 25 Aug 2017, a group of 15 adults and elementary school students from the same church in Jeonju visited McDonald’s Jeonju branch and ate bulgogi burgers together. A day later, 7 children and 1 adult experienced diarrhea and vomiting. The authorities declared an epidemiological probe into the case on Sat 2 Sep 2017.

As the case was publicized, McDonald’s Korea halted selling bulgogi burgers nationwide starting on Sat 2 Sep 2017. On its website, a notice says, “We have decided to stop selling bulgogi burgers pending the outcome of the ongoing investigation by authorities,” adding that the multinational company’s Korean branch was taking the issue seriously. It also said the company will do its best to help patients recover, though without elaborating on how or when.

The outcome of the ongoing probe is expected to be made public around Wed 6 Sep 2017.

ProMED-mail reports classic bulgogi recipes use a marinade of soy, ginger, garlic, and gochujang (Korean chili paste) to flavor and tenderize strips of meat. Because combining a marinade into ground beef would change the consistency of the hamburger, making it more of a “sloppy-joe”, usually a bulgogi inspired sauce is used on top of the burger although a Korean inspired spice combination can be added to the ground beef.

NZ says (not a joke): Have your say on cooking burgers: Until a chef offers a temperature rather than adjectives it’s bullshit

While Australia is being dragged kickin-and-screamin into the thermometer age, New Zealand has decided to put knowledge aside, and ask the people, How do you want your burgers done?

Just because everyone eats doesn’t mean they know anything about microbial food safety.

The NZ Ministry for Primary Industries issued a public notice, stating: Feedback from chefs is that they would like to be able to cook mince (especially minced burger patties) to medium/medium rare (I have no idea what these adjectives mean; some numbers, please) under the template food control plan.

MPI has worked with chefs, environmental health offers and food scientists to develop a specialist section for both official template food control plans.  The specialist page is written in the “Know, Do, Show” format from the Simply Safe & Suitable template. The section will allow red meat mince for medium/medium rare burgers, and other meat specialities like steak tartare, to be safely served lightly cooked or raw. (Carpaccio is already covered in the templates (refer to section 10.6 (Serve) –  Whole cuts and whole joints of meat – and the ‘Cooking food’ page in Simply Safe & Suitable).

We want to know if the specialist section works for you? Have we got it right?  

Please note: Two of the processes included in the consultation are sanitising and blanch-in-a-bag.  The scientific validation for these methods is ongoing.  If there is insufficient evidence for it to be included in this amendment for the official template food control plans, and there is high demand for the process, further research would need to be commissioned so it could be added at a later date.

The consultation opens 25 July and closes on 8 August 2017.

Email your feedback to foodact.2014@mpi.govt.nz

I suggest e-mailing them this blog post and saying, I want a burger cooked safely to a verified 74C.

Use a thermometer Ireland pt, deux: Growing trend for eating rare burgers could hide deadly bacteria

Gavin White of the Independent follows up on the warning from safefood Ireland that there is “no way of knowing” if rare burger meat is safe.

A leading food safety expert said he was “very surprised” restaurants were offering undercooked burgers and putting their customers at risk.

Professor Martin Cormican, from the school of Medicine in NUI Galway, said small children and pregnant women were at an even higher risk of becoming ill.

“Restaurants need to understand that not every customer is the same and some are at more risk than others. There are liability issues,” Prof Cormican said.

He said that every burger had the potential to have the deadly bacteria, Vtec, which could cause severe illness.

“Although steak can have its bacteria killed on the outside, mince has the potential for the bacteria to end up in the middle where if not cooked properly, has the potential to make you seriously ill,” he said.

Safefood Ireland has launched its Burger Fever campaign as it was revealed 96pc of Irish people consider themselves well informed about food safety, yet 51pc are eating undercooked burgers.

A batch of French mince was recalled last week from French supermarkets over worries for the presence of Vtec, and Prof Cormican said it could easily happen in Ireland.

“Don’t take the risk, and especially if you’re taking medicine for illnesses like rheumatoid arthritis which severely impacts your immune system,” Prof Cormican said.

Dr Linda Gordon, chief specialist in food science at Safefood, said around 2pc of all mince had Vtec in it so the risk was always there for the “growing trend” of burger lovers.

Assessment of risk communication about undercooked hamburgers by restaurant servers

Ellen M. Thomas, RTI International; Andrew Binder, Anne McLaughlin, Lee-Ann Jaykus, Dana Hanson, and Benjamin Chapman, North Carolina State University; and Doug Powell, barfblog.com

Journal of Food Protection

DOI: 10.4315/0362-028X.JFP-16-065

According to the U.S. Food and Drug Administration 2013 Model Food Code, it is the duty of a food establishment to disclose and remind consumers of risk when ordering undercooked food such as ground beef. The purpose of this study was to explore actual risk communication activities of food establishment servers. Secret shoppers visited restaurants (n=265) in seven geographic locations across the U.S., ordered medium rare burgers, and collected and coded risk information from chain and independent restaurant menus and from server responses. The majority of servers reported an unreliable method of doneness (77%) or other incorrect information (66%) related to burger doneness and safety. These results indicate major gaps in server knowledge and risk communication, and the current risk communication language in the Model Food Code does not sufficiently fill these gaps. Furthermore, should servers even be acting as risk communicators? There are numerous challenges associated with this practice including high turnover rates, limited education, and the high stress environment based on pleasing a customer. If it is determined that servers should be risk communicators, food establishment staff should be adequately equipped with consumer advisory messages that are accurate, audience-appropriate, and delivered in a professional manner so as to help their customers make more informed food safety decisions.

 

Use a fucking thermometer: Ireland says eating undercooked burgers ‘like driving without a seatbelt’

Diners who enjoy a juicy burger have been warned that eating them pink in the middle is “like driving without wearing a seatbelt”.

Fiachradh McDermott of The Irish Times reports that safefood began its new campaign, “Burger Fever”, on Thursday to inform people that eating undercooked burgers could lead to serious or sometimes life-threatening food poisoning.

What she didn’t report is that color is a terrible indicator of safety, and that needle-or-blade-tenderized steaks carry the same risk as mince, so this science-based agency is publishing fairy tales.

But I’ll let the bureaucrats speak for themselves and you judge.

The body advised people to always ask for burgers to be well-cooked in restaurants.

In an online survey conducted by safefood, 96 per cent of people considered themselves well-informed about food safety. However, 51 per cent admitted to eating undercooked burgers.

Two thirds of respondents said they would reconsider their choice if they knew there was a possibility of food poisoning.

Undercooked burgers carry the risk of E. coli, which can have long-term effects. The biggest worry is a type called VTEC, which causes severe diarrhoea.

Dr Linda Gordon, chief specialist in food science at safefood, said it can result in “frequent serious complications.” VTEC can affect the blood and kidneys, and is most serious in older people and children under five. However, it only takes as little as ten E. coli cells to make a person sick, she said.

Dr Gordon said the campaign is intended as a preventative measure, but “emphasising the difference between a burger and a steak” is an important aspect.

According to Dr Gary Kearney, director of food science at safefood, “Mince used in hamburgers is a higher risk as the food poisoning bacteria that live on the surface of the beef (steak) is then mixed through the middle of the burger when the beef is minced – so in effect, the outside is now on the inside.”

Dr Martin Cormican, Professor of Bacteriology at National University of Ireland, Galway (NUIG), emphasised the possibility and danger of contracting VTEC from undercooked meat.

“Eating burgers that are pink in the middle is a bit like driving without a seatbelt; you might get away with it for years but if something goes wrong and you are harmed, will you still think it was worth it?”

No mention of thermometer, cooking temps and hold times, just plain pandering.

Use a fucking thermometer and stick it in.

Assessment of risk communication about undercooked hamburgers by restaurant servers

E. Thomas, E,M,, Binder, A., McLaughlin, A., Jaykus, L., Hanson, D, Powell, D.A., Chapman, B. 2016.

Journal of Food Protection Vol. 79, No. 12, pp. 2113-2118

http://www.jfoodprotection.org/doi/abs/10.4315/0362-028X.JFP-16-065?code=fopr-site

According to the U.S. Food and Drug Administration 2013 Model Food Code, it is the duty of a food establishment to disclose and remind consumers of risk when ordering undercooked food such as ground beef.

The purpose of this study was to explore actual risk communication activities of food establishment servers. Secret shoppers visited restaurants (n=265) in seven geographic locations across the U.S., ordered medium rare burgers, and collected and coded risk information from chain and independent restaurant menus and from server responses.

The majority of servers reported an unreliable method of doneness (77%) or other incorrect information (66%) related to burger doneness and safety. These results indicate major gaps in server knowledge and risk communication, and the current risk communication language in the Model Food Code does not sufficiently fill these gaps. Furthermore, should servers even be acting as risk communicators? There are numerous challenges associated with this practice including high turnover rates, limited education, and the high stress environment based on pleasing a customer. If it is determined that servers should be risk communicators, food establishment staff should be adequately equipped with consumer advisory messages that are accurate, audience-appropriate, and delivered in a professional manner so as to help their customers make more informed food safety decisions.

Poison customers, it’s good for business: Burger row hits the grills in New Zealand; try a thermometer

New Zealand’s oldest licensed premises has pulled a burger that’s been the cornerstone of its menu – blaming it on bureaucratic red tape gone mad.

Dan Fraser, executive chef at the Duke of Marlborough restaurant in the Bay of Islands, was left stewing after a visit from a Ministry of Primary Industries inspector on Thursday. 

Nicole Lawton of The Sunday Star Times reports new food preparation guidelines from MPI state minced meat and liver needs to be cooked at high temperatures for a longer amount of time than previously, to avoid contamination. 

Fraser said the new rules were a raw deal and will now prevent him serving his signature burger The Governor’s Burger which is pink and juicy in the middle. 

The Governor’s Burger features bacon, cheese, pickle, tomato, chipotle mayonnaise and a medium rare beef mince patty.

“It’s a really good burger, we really pride ourselves in presenting it to our customers,” Fraser said.

“Basically, the ministry is telling us how our customers need to eat their food.”

MPI food and beverage manager Sally Johnston, said the new rules didn’t entirely ban medium-rare meat – but chefs would have to change how they cooked it.

“If they do want to serve a medium-rare burger, it is possible, it just might take a little more forethought and planning,” Johnston said.

“It is possible to cook a medium-rare burger safely, it just means that they need to think about the processes that they are using to do that. It might not be necessarily possible to do that on a BBQ or grill.”

She suggest sous vide methods of cooking instead – what people used to call boil-in-the-bag.

“Who the f*** wants a sous vide burger?”, Fraser said.

The new rules state meat should have an internal minimum temperature of 65°C for 15 minutes while cooked, 70°C for three minutes, or 75°C for 30 seconds.

But Fraser said those were rules drawn up by a bureaucrat and not a chef. They meant a beef mince patty would always be “rubbery and devoid of flavour”.

Johnston insists the new rules are necessary. “People have died from under cooked burgers, there is a genuine food safety risk here, we’re not doing this to take the fun out of food. Bugs that have caused people to die (such as E. coli) are frequently found in New Zealand meat.”

The new MPI guidelines detail how restaurants and food businesses should prepare, store and serve their food, and supplement the 2014 Food Act. 

Top chef Ray McVinnie told Stuff NZ that serving a medium-rare burger is “dangerous and dumb” and that any chef who complains about such regulations does not understand basic food safety.

Yesterday, the Ministry for Primary Industries decided they will be talking to chefs about ways they can serve medium rare burgers and still keep food safe for consumers.

“We’re happy to work with chefs wanting to develop a custom Food Control Plan that covers their specific menu items. It might need different methods of sourcing, storing, and handling meat to make sure consumers are still protected.”

The move by MPI to regulate chefs’ kitchens brought howls of outrage and ridicule from those interviewed by the NZ Herald.

Labour’s Damien O’Connor said it was “ridiculous overkill”.

“We’ve got strict controls on how you kill and process meat. To then look at the cooking of it is nanny-state gone mad.

Northland MP Winston Peters, who has eaten at the Duke of Marlborough often over the years, said “paternalistic bureaucrats” were killing New Zealand businesses.

Sick customers ruin biz.

I look forward to the microbiologically-based arguments the talking heads will bring to the public discussion.

Assessment of risk communication about undercooked hamburgers by restaurant servers

Ellen M. Thomas, RTI International; Andrew Binder, Anne McLaughlin, Lee-Ann Jaykus, Dana Hanson, and Benjamin Chapman, North Carolina State University; and Doug Powell, powellfoodsafety.com

Journal of Food Protection

DOI: 10.4315/0362-028X.JFP-16-065

According to the U.S. Food and Drug Administration 2013 Model Food Code, it is the duty of a food establishment to disclose and remind consumers of risk when ordering undercooked food such as ground beef. The purpose of this study was to explore actual risk communication activities of food establishment servers. Secret shoppers visited restaurants (n=265) in seven geographic locations across the U.S., ordered medium rare burgers, and collected and coded risk information from chain and independent restaurant menus and from server responses. The majority of servers reported an unreliable method of doneness (77%) or other incorrect information (66%) related to burger doneness and safety. These results indicate major gaps in server knowledge and risk communication, and the current risk communication language in the Model Food Code does not sufficiently fill these gaps. Furthermore, should servers even be acting as risk communicators? There are numerous challenges associated with this practice including high turnover rates, limited education, and the high stress environment based on pleasing a customer. If it is determined that servers should be risk communicators, food establishment staff should be adequately equipped with consumer advisory messages that are accurate, audience-appropriate, and delivered in a professional manner so as to help their customers make more informed food safety decisions.

Color sucks: Use a thermometer and stick it in for food safety

safefood Ireland has joined the UK Food Standards Agency in providing terrible advice about how to cook burgers.

A recipe for summer beef burgers (may a fine solstice greet our Northern and Southern friends) endorsed by safefood says:

“Before serving, ensure that the burgers are cooked thoroughly. Cut into them with a clean knife and check that they are piping hot all the way through, there is no pink meat remaining and that the juices run clear.”

Meanwhile, FSA issued a Safe Summer Food guide as UK picnickers head out in the sun (there’s sun in the UK?). The guidelines were in part based results of a self-reported survey, which is largely meaningless but something FSA likes to do.

The Morning Advertiser has more details on the hoops FSA seems willing to jump through to ensure the safety of rare burgers including:

  • sourcing the meat only from establishments which have specific controls in place to minimise the risk of contamination of meat intended to be eaten raw or lightly cooked;
  • ensuring that the supplier carries out appropriate testing of raw meat to check that their procedures for minimising contamination are working;
  • Strict temperature control to prevent growth of any bugs and appropriate preparation and cooking procedures;
  • notifying their local authority that burgers that aren’t thoroughly cooked are being served by the business; and,
  • providing advice to consumers, for example on menus, regarding the additional risk.

The advice from these self-proclaimed science-based agencies is at odds with, uh, science.

It has been known for over two decades that color is a lousy indicator of safety in hamburger.

The latest addition to this work comes from Djimsa et al. in the Dept. of Animal Science at Oklahoma State Univ., who wrote in the Journal of Food Science earlier this year that:

Premature browning is a condition wherein ground beef exhibits a well-done appearance before reaching the USDA recommended internal cooked meat temperature of 71.1 °C; however, the mechanism is unclear.

The objectives of this study were: (1) to determine the effects of packaging and temperature on metmyoglobin reducing activity (MRA) of cooked ground beef patties and (2) to assess the effects of temperature and pH on thermal stability of NADH-dependent reductase, lactate dehydrogenase (LDH), and oxymyoglobin (OxyMb) in-vitro.

Beef patties (lean: fat = 85:15) were packaged in high-oxygen modified atmosphere (HiOX-MAP) or vacuum (VP) and cooked to either 65 or 71 °C. Internal meat color and MRA of both raw and cooked patties were determined. Purified NADH-dependent reductase and LDH were used to determine the effects of pH and temperature on enzyme activity. MRA of cooked patties was temperature and packaging dependent (P < 0.05). Vacuum packaged patties cooked to 71 °C had greater (P < 0.05) MRA than HiOX-MAP counterparts.

Thermal stability of OxyMb, NADH-dependent reductase, and LDH were different and pH-dependent. LDH was able to generate NADH at 84 °C; whereas NADH-dependent reductase was least stable to heat.

The results suggest that patties have MRA at cooking temperatures, which can influence cooked meat color.

Effects of metmyoglobin reducing activity and thermal stability of NADH-dependent reductase and lactate dehydrogenase on premature browning in ground beef

Journal of Food Science, 2017 Feb, 82(2):304-313, doi: 10.1111/1750-3841.13606. Epub 2017 Jan 18.

https://www.ncbi.nlm.nih.gov/pubmed/28099768

1 dead, 29 sick from E. coli O157 linked to minced meat in Germany

We report an ongoing, protracted and geographically dispersed outbreak of hemolytic uremic syndrome (HUS) and gastroenteritis in Germany, involving 30 cases since December 2016. The outbreak was caused by the sorbitol-fermenting immotile variant of Shiga toxin-producing (STEC) Escherichia coli O157.

Molecular typing revealed close relatedness between isolates from 14 cases. One HUS patient died. Results of a case–control study suggest packaged minced meat as the most likely food vehicle. Food safety investigations are ongoing.

Ongoing hemolytic uremic syndrome (HUS) outbreak caused by sorbitol-fermenting (SF) shiga toxin-producing Escherichia coli (STEC) O157, Germany, December 2016 to May 2017

Eurosurveillance, vol. 22, issue 21, 25 May 2017, S Vygen-Bonnet, B Rosner, H Wilking, A Fruth, R Prager, A Kossow, C Lang, S Simon, J Seidel, M Faber, A Schielke, K Michaelis, A Holzer, R Kamphausen, D Kalhöfer,S Thole , A Mellmann, A Flieger, K Stark

http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=22805