Still need a better descriptor: Cross-contamination and cutting boards

Cross-contamination is one of the main factors related to foodborne outbreaks. This study aimed to analyze the cross-contamination process of Salmonella enterica serovar Enteritidis from poultry to cucumbers, on various cutting board surfaces (plastic, wood, and glass) before and after washing and in the presence and absence of biofilm.

Thus, 10 strains of Salmonella Enteritidis were used to test cross-contamination from poultry to the cutting boards and from thereon to cucumbers. Moreover, these strains were evaluated as to their capacity to form biofilm on hydrophobic (wood and plastic) and hydrophilic materials (glass).

We recovered the 10 isolates from all unwashed boards and from all cucumbers that had contacted them. After washing, the recovery ranged from 10% to 100%, depending on the board material. In the presence of biofilm, the recovery of salmonellae was 100%, even after washing. Biofilm formation occurred more on wood (60%) and plastic (40%) than glass (10%) boards, demonstrating that bacteria adhered more to a hydrophobic material.

It was concluded that the cutting boards represent a critical point in cross-contamination, particularly in the presence of biofilm. Salmonella Enteritidis was able to form a biofilm on these three types of cutting boards but glass showed the least formation.

Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards

01.feb.18

Foodborne Pathogens and Deases, Volume 15, No. 2

Dantas Stéfani T. A. , Rossi Bruna F. , Bonsaglia Erika C. R. , Castilho Ivana G. , Hernandes Rodrigo T. , Fernandes Ary Júnior, and Rall Vera L. M.

https://doi.org/10.1089/fpd.2017.2341

Food Safety Talk 81: Food safety matters every week

Food Safety Talk, a bi-weekly podcast for food safety nerds, by food safety nerds. The podcast is hosted by Ben Chapman and barfblog contributor Don Schaffner, Extension Specialist in Food Science and Professor at Rutgers University. Every two weeks or so, Ben and Don get together virtually and talk for about an hour.  They talk about what’s on their minds or in the news regarding food safety, and popular culture. They strive to be relevant, funny and informative — sometimes they succeed. You can download the audio recordings right from the website, or subscribe using iTunes.

After a brief discussion about Quadrophenia, the guys thankfully decide to not sing this episode.Unknown-3

Ben mentions that the last video store in the Raleigh area is closing. This led to some discussion about the job security of academic careers where Don stated, ‘prediction is very difficult especially about the future.’

Spurred by Ben’s short visit to Baltimore, the guys then discuss how awesome The Wire is.  Don mentions a perspective by David Simon, the Wire’s creator, on the real life situation in Baltimore.  Ben was recently in Baltimore for the Food Safety Summit.  A nod goes out to Brian Saunders for doing a good job of boots on the ground coverage of what’s going on in Baltimore during the Food Safety Summit.

Don recommends Acorn TV for anyone interested in British TV. This subscription service has British programming not typically shown on US TV. At the Acorn website Ben spotted Time Team an archeology reality series that he thinks his kids would love.

This week Ben talks about media interviews and a focus on multiple food safety stories all hitting at the same time. He talked a cutting boards post on barfblog that garnered some attention.  He also fielded inquires regarding the Blue Bell Listeria outbreak .  Ben noted that Blue Bell announced they are recalling all the ice cream.

A tragic botulism outbreak linked to a church potluck in Ohio was also a topic in multiple media outlets. The potluck outbreak was linked to home-canned potatoes but the coverage prompted a side conversation about bot and foil-wrapped baked potatoes.

Looking ahead to future food outbreaks Ben mentions that a bill was introduced in North Carolina to legalize raw milk.  This bill would allow consumers to legally acquire raw milk via a cow share mechanism.  In this article Ben is quoted challenging an inappropriate comparison of raw milk outbreak data by the bill’s sponsor.

In After Dark Don shames Ben for not listening to Roderick on the Line. Again.

– 30 –

With a check by Schaffner: How to avoid food poisoning at home

Science is about disagreements, revising knowledge and generating new evidence-based knowledge (someone will disagree with that).

Don-Schaffner-214x300Don Sapatkin of the Philadelphia Inquirer recently asked a number of food safety types about food safety at home. For fun, I asked friend of the barfblog and known bugcounter, Don Schaffner of Rutgers University (left, prettymuch as shown) his thoughts on the answers.

“Washing a sponge with soap doesn’t get rid of bacteria,” said microbiologist Michael Doyle, director of the University of Georgia’s Center for Food Safety (below, right). They grow at room temperature and get spread around anything else you wipe off. Put the sponge in a microwave for one minute to kill the salmonella and other bacteria,” he said.

Schaffner: Sort of true. Washing a sponge will probably remove some bacteria, but not all. Same with the microwave: it depends upon the microwave, the amount of moisture in the sponge, etc. A better practice may be to put your sponges in the automatic dishwasher, assuming you have one.

 Experts say most home kitchens are far dirtier.

Schaffner: Might be true, but science-based head-to-head comparisons are lacking.

 Cutting boards should not have hard-to-clean nicks and grooves (wood is better, Doyle said, because the resin has antibacterial properties).

Schaffner: Dean Cliver’s work showed wooden cutting boards to be safer, but the literature is far from clear on the matter.

 Washing chicken in the sink may sound hygienic but actually poses all sorts of risks.

Schaffner: Yup, this has good scientific consensus.

 “Every time you run your disposal in the sink you are generating a little airflow back up.”

michael.doyle.produce.07Schaffner: Yup, probably true.

 If you do wash chicken in the sink, clean it (the sink) with bleach (1 ounce in 1 gallon of water).

Schaffner: Giving bleach concentration recommendations always concerns me. The units are never the same, the knowledge about the type of bleach is never certain, and the type of surface being cleaned makes a difference (plates versus countertops).  I used to dream of creating a webpage that would definitively answer these questions, and do unit conversions. Now I have the same dream except it’s an iPhone app.

Craig Hedberg, a professor at the University of Minnesota School of Public Health (below, left): “If you take this big mass of hot food and put it into a plastic container and put a lid on it, you are holding the heat in and slowing the cooling process, even if you put it in the refrigerator. You want to get it out of bacterial-growth range” – 40 to 140 degrees – “within a couple of hours.” Pouring it into containers no more than four inches deep speeds the process.

Schaffner: This is more or less correct, but I believe the correct depth of the food recommendation is 3 inches. It doesn’t really matter how deep the container is, it’s the depth of the food.

If food is not cooled fast enough, spores that survived cooking can germinate and grow bacteria. Reheating leftovers to 165 degrees for 15 seconds will kill them.

Schaffner: This is the general time temperature recommendation. I’ve never checked to see what log reduction it would give for Clostridium perfringens cells, but it’s likely sufficient.

Hedberg advises against washing prewashed bagged lettuce; E. coli and salmonella can adhere to cut surfaces and tiny pores. “If it’s contaminated, your washing it again would not eliminate the contamination,” he said. “If it is not contaminated, your washing may contaminate it.”

Schaffner: That is consistent with expert recommendations.

 Hands should be washed vigorously with soap before preparing food or eating; after handling raw meat, poultry, fish, or even raw produce; and after smoking, eating, or drinking.

Craig HedbergSchaffner: Also after pooping or changing a diaper, handling pets etc.

 Countertops, cutting boards, utensils, etc., should be cleaned with hot water after every use.

Schaffner: I also recommend soap.

 Cooking and holding temperatures should be checked, which means having working thermometers. (The fridge should be set between 32 and 40 degrees Fahrenheit; the freezer, at zero or below).

Schaffner: They nailed this one.

 Everything should be clean: Garbage covered, or at least three feet from food-preparation areas; pets never allowed in the kitchen (and hands washed after petting).

Schaffner: I’m not sure where the three-foot recommendation comes from, and it’s probably not science-based. Does anyone really exclude their pets from the kitchen? When we had dogs their food and water dishes were in the kitchen. Good luck getting a cat to do anything you want to do.

To bring home cooks up to speed, the Rutgers University Cooperative Extension posts a quick home kitchen food safety best practices check-Up list: http://bit.ly/1xDO19F.

Is there a better name for cross-contamination: kitchen cutting boards remain a source of multidrug-resistant bacteria after use

After handling raw poultry, hands of food preparers and cutting boards remain a source of transmission for multi-drug resistant bacteria, such as E. coli that produce extended-spectrum beta-lactamases (ESBLs). The study of household and hospital kitchens was published in the May issue of the journal Infection Control and Hospital Epidemiology.

Dan Aykroyd Plays Julia Child“The spread of multidrug-resistant bacteria has been associated with the hospital setting, but these findings suggest that transmission of drug-resistant E. coli occurs both in the hospital and households,” says Andreas Widmer, MD, lead author of the study. “Our findings emphasize the importance of hand hygiene, not only after handling raw poultry, but also after contact with cutting boards used in poultry preparation.”

Researchers from University Hospital in Basel, Switzerland collected and examined 298 cutting boards (154 from University Hospital and 144 from private households) after preparation of various meats (i.e., poultry, beef/veal, pork, lamb, game and fish) and before being cleaned. They also collected 20 pairs of gloves from hospital kitchen employees after they handled raw poultry. These samples were tested for the presence of ESBL-producing Enterobacteriaceae, a family of gram-negative bacteria that includes Salmonella, E. coli and Klebsiella.

In testing the cutting boards, researchers found that 6.5 percent of hospital cutting boards used in preparation of poultry were contaminated with ESBL-producing E. coli. For boards used in households, researchers found ESBL-producing E. coli cutting.board.chicken.nov.13on 3.5 percent of these surfaces. They also found that 50 percent of the hospital kitchen gloves were contaminated with this drug-resistant E. coli.

The researchers found that none of the cutting boards used in preparing beef/veal, pork, lamb, game or fish were contaminated with any ESBL-producing bacteria. They also found that the meat’s country of origin did not play a factor in the presence of bacteria on any of the surfaces.

Reference: Sarah Tschudin-Sutter, Reno Frei, Roger Stephan, Herbert Hächler, Danica Nogarth and Andreas F. Widmer. “Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae – a threat from the kitchen.” Infection Control and Hospital Epidemiology [35:5] (May 2014).

Source: Society for Healthcare Epidemiology of America (SHEA)