Be the bug, easy to figure out: Charges laid over NZ gastro outbreak

In August, 2016, 5,200 people were sickened with Campylobactor  after the Havelock North, NZ, water supply was contaminated.

vomit-dontLast week, the Hawke’s Bay District Health Board’s public health unit followed up reports of a gastro illness circulating in the community.

On Nov. 18, 2016 Hawke’s Bay Regional Council laid charges against a party for alleged offences uncovered in the course of its investigation into the contamination of Havelock North drinking water.

The charges were laid after the council investigated the source of the contamination that resulted in more than 5000 people getting sick, and the condition of water supply bores in the area.

The council said its investigations had found evidence of a breach of the maintenance conditions of the party’s resource consent. If a breach was proved, the resource consent no longer permitted the taking of water.

The council has commenced a prosecution against the party, alleging the unlawful taking of water from the aquifer arising from the alleged failure to meet well head maintenance conditions.

Council chief executive Andrew Newman said the drinking water contamination has had a devastating effect on the Havelock North community with wider regional impacts and the council was “very keen to see the cause of the contamination identified and to ensure it does not happen again”.  

He said his council had more than 15 people working on its investigations.

These included council scientists, and Environmental Science Research (ESR) with expertise in the environment, land use, water and climate, as well as dye tracing experts.

He said their investigations had included surface and groundwater quality, the bore infrastructure, water pathways in the local environment and livestock in nearby paddocks.

Michael Pollan didn’t invent microbiome research, he’s a demagogue: Set of 15 bacterial species protects mice from Salmonella as effectively as natural gut microbiota

The mammalian gut harbors thousands of microbial species – collectively known as the microbiota or microbiome – that interact with each other and with their host to form a complex ecosystem.

lmu-set-of-15-bacterial-species-protects-mice-from-salmonella-infections-as-effectively-as-does-the-natural-gut-microbiotaIn healthy organisms, this community provides an effective shield against infection by many pathogenic organisms, such as Clostridium difficile (which is responsible for antibiotic-associated diarrhea) and various Salmonella species.

Researchers led by LMU microbiologist Professor Bärbel Stecher, in cooperation with colleagues from the University of Vienna and the Technical University of Munich, now show that, in the mouse, a defined group of 15 bacterial species confers the same degree of protection against Salmonella infections as does the host’s natural microbiota. The work establishes a new model system for the investigation of the interaction between the gut microbiome and infectious pathogens, which could in turn provide new approaches to the treatment of gastrointestinal infections. The new findings appear in the journal Nature Microbiology.

The protective effect provided by the gut microbiota against infection by invasive pathogens is referred to as colonization resistance. Exposure to antibiotics can disrupt this mechanism because these drugs typically alter the composition of the bacterial population in the gastrointestinal tract. “However, the contribution made by individual bacterial species to colonization resistance remains unclear,” says Stecher, who is also member of the German Center for Infection Research (DZIF).

“In order to gain a better understanding of the functions of the gut microbiota in this context, we had already established in my laboratory a minimal consortium comprising 12 bacterial species which are representative for the gut microbiome of the mouse.” This set of species, which is referred to as Oligo-MM-12, can be introduced into germ-free mice and is stably maintained over several generations. However, while mice colonized by the Oligo-MM-12 species are more resistant to infection by Salmonella enterica than their germ-free relatives, they are not as well protected as mice with a normal microbiome.

pollan-microbiomeThe team then went on to develop a new strategy, called genome-guided microbiota design, to identify species required to confer the same measure of protection as the natural gut microbiome of the mouse.

“We compared DNA sequences from the 12 species represented in Oligo-MM-12 with homologous sequences derived from the total mouse microbiome, and were able to identify groups of genes that were missing from our set,” Stecher explains. Some of these genes turned out to be characteristic for so-called facultative anaerobes, i.e. bacterial species that grow best in the presence in oxygen, but are nevertheless capable of proliferating in its absence. Indeed, the genus Salmonella consists of facultative anaerobes, while almost all the species that make up the Oligo-MM-12 consortium are obligate anaerobes – for which oxygen is toxic.

“We therefore supplemented our original consortium with three facultatively anaerobic species that are found in the microbiota of healthy mice,” Stecher says, “and we were able to demonstrate experimentally that this combination confers the same level of colonization resistance against Salmonella as that observed in mice that have a natural microbiota.” Stecher and her colleagues believe that their new “mini-microbiota”, together with the use of genome-guided microbiota design, provides a powerful new tool for the identification of hitherto unknown functions mediated by natural microbiota. This opens a route to the identification of specific bacterial species that could ameliorate the effects of disease-dependent dysfunction of the gut microbiota.

FDA says 1 percent of US cucumbers carry Salmonella

One in a hundred cucumbers carries Salmonella, according to new data from the U.S. Food and Drug Administration — and for fresh hot peppers that number rises to three per hundred.

cucumber-spainmepBoth the vegetables were targeted by the agency’s proactive testing because of their role in previous outbreaks. Because cucumbers are often eaten raw, bacteria on them are more likely to make it into food; raw cucumbers have been blamed in five outbreaks of illness from 1996 to 2014.

Hot peppers, such as jalapeño and serrano peppers, on the other hand, are often cooked but can be a “stealth component” of multi-ingredient dishes, the FDA said. In 2008, hot peppers were implicated in an outbreak that caused 1,500 illnesses, 308 hospitalizations, and two deaths.

The FDA’s proactive sampling program began testing for disease-causing microbes in certain foods in 2014 to learn more about the prevalence of disease-causing bacteria and to help the agency identify patterns that may help predict and prevent future contamination.

The latest findings, released lastThursday, included results from 1,050 cucumber samples and 1,130 hot pepper samples. Eventually 1,600 of each will be sampled.

Of the cucumber samples, 15 tested positive for salmonella. None tested positive for E. coli. Of the hot pepper samples, 35 tested positive for salmonella, and one tested positive for a strain of Shiga toxin-producing E. coli that was determined to be incapable of causing severe illness.

The samples were collected at ports, packing houses, manufacturers, and distributors across the US.

The agency may take enforcement action, such as a recall, on foods that test positive.

“This testing is still underway and no conclusions can be drawn at this time,” according to the FDA.

 In 2014, the FDA started a sampling program for a variety of commodities to learn more about the prevalence of disease-causing bacteria on the commodities.

 The microbiological sampling assignments were designed to collect a statistically determined number of samples of certain commodities over 12 to 18 months and test them for certain types of bacteria that can cause foodborne illnesses.

USDA: Everything you ever wanted to know about labeling needle- or blade–tenderized beef

Compliance Documents

Q1. Where can I find information on the new “mechanically tenderized beef products regulation per 9 CFR 317.2(e)(3)?

Information on “mechanically tenderized beef products” is available from the following locations:

Labeling Issues

Q2. Under this final rule, will the product need to be labeled with the specific method of mechanical tenderization used to prepare the product?

tenderizedmeat2_custom-949f4ddbfc4f2cb411923f9296e69966fe69d995-s1100-c85No, the label need not include the specific type of mechanical tenderization used. To provide flexibility, FSIS is allowing the phrase ‘‘mechanically tenderized’’ to be used as the descriptive designation on any type of mechanically tenderized product. In addition, in lieu of “mechanically tenderized,” such product may be labeled as ‘‘needle tenderized’’ or ‘‘blade tenderized,’’ as applicable.

Q3. Can “needle injected” be used as the descriptive designation on the labels of raw or partially cooked beef products that have been mechanically tenderized?

No, needle injected may not be used as the descriptive designation. The terms “needle tenderized” or “mechanically tenderized” must be used as the descriptive designation for needle tenderized raw or partially cooked beef products and the terms “mechanically tenderized” or “blade tenderized” must be used as the descriptive designation for raw or partially cooked blade tenderized beef products.

Q4. Are the descriptive designations “mechanically tenderized,” “blade tenderized,” or “needle tenderized” only required on raw or partially cooked beef products?

Yes, unless the product is destined to be fully cooked or to receive another full lethality

treatment at an official establishment, such product must be labeled accordingly.

Q5. Do the new labeling requirements apply to mechanically tenderized pork, lamb, or goat products?

No. The rule applies only to raw or partially cooked beef products that have been mechanically tenderized.

Q6. Can establishments put both mechanically tenderized beef products and non- mechanically tenderized beef products in the same immediate container and label it with the descriptive designation “mechanically tenderized?”

No. To label product as “mechanically tenderized” when it was not would be false and misleading.

needle-tenderize-crQ7. If we sell mechanically tenderized raw or partially cooked beef or veal products in protective coverings, must the protective coverings meet the mechanical tenderization labeling requirements when the immediate container of this product is labeled “For Institutional Use Only?”

No. Under 9 CFR 317.1(a)(1), protective coverings should not bear any mandatory labeling information.” In this case, the immediate container, which also serves as the shipping container, is required to be labeled with the descriptive designation and bear validated cooking instructions and all other applicable labeling features.

Q8. Is beef cubed steak is subject to the new labeling requirements?

No, this regulation will not apply to raw or partially cooked beef products that have been cubed. The regulation is specific to needle and blade tenderized beef products. FSIS stated in the final rule:

The descriptive designation will only apply to raw or partially cooked beef products that have been needle tenderized or blade-tenderized, including beef products injected with marinade or solution. Other tenderization methods, such as pounding and cubing, change the appearance of the product, putting consumers on notice that the product is not intact. Moreover, most establishments already label cubed products as such. (80 FR 28157)

Q9. Must the labels for raw or partially cooked mechanically tenderized beef products be submitted to the FSIS Labeling and Program Delivery Staff (LPDS) for approval?

No. The descriptive designations, “mechanically tenderized,” “blade tenderized,” and “needle tenderized” are not considered special statements or claims under 9 CFR 412.1(c). Therefore, as stated in the final rule, simply adding the descriptive designation and validated cooking instructions to a label would not require LPDS approval, given the label is otherwise in accordance with FSIS’s regulations.

Q10. Do the new labeling requirements apply to raw or partially cooked mechanically tenderized beef products that are produced at establishments that use a validated intervention during the production of such products?

Yes, the new labeling requirements would apply to products treated with a validated antimicrobial intervention, unless the establishment applies a lethality treatment that achieves a 5-log reduction in pathogens. Mechanically tenderized beef product treated at an official establishment with an intervention or process, including HPP, that has been validated to achieve at least a 5-log reduction for Salmonella and Shiga Toxin-producing E. coli (STEC) organisms (including E. coli 0157:H7) would not be subject to the requirements in this final rule because it has received a full lethality treatment. (See 80 FR 28153)

Q11. Do the new labeling requirements apply to mechanically tenderized beef products labeled or prepared at retail stores?

Yes, the new labeling requirements would apply to raw or partially cooked mechanically tenderized beef products produced, packaged, and labeled at a retail store.

Cooking Instructions

Q12. Is there compliance guidance available on validating cooking instructions for mechanically tenderized beef products?

Yes, at:

FSIS Compliance Guideline for Validating Cooking Instructions for Mechanically Tenderized Beef Products

Q13. Where can I find scientific studies on validated cooking instructions?

Attachment 1 of the above FSIS Compliance Guideline for Validating Cooking Instructions for Mechanically Tenderized Beef Products contains a summary of published scientific support for cooking instructions.

Q14. Do the new labeling requirements apply to raw or partially cooked mechanically tenderized beef products that are too thin to practically measure their internal temperature using a food thermometer?

No, the new labeling requirements do not apply to raw or partially cooked mechanically tenderized (including through injection with a solution) beef products that are too thin to measure their internal temperature using a food thermometer, such as beef bacon or carne asada. FSIS does not intend to enforce the requirements for these products because they are customarily prepared in a manner that is sufficient to destroy pathogenic bacteria.

Note that the thickness of many food thermometers used by consumers is approximately 1/8,” making it difficult to measure the end product temperature of products 1/8” thick or less through use of a thermometer.

Q15. Where on the label of raw or partially cooked mechanically tenderized beef products can the validated cooking instructions appear?

Validated cooking instructions must appear on the immediate containers of all raw or partially cooked mechanically tenderized beef products destined for household consumers, hotels, restaurants, or similar institutions. These instructions can appear anywhere on the product label.

Mechanically Tenderized Beef With Solutions

Q16. Must the label of a raw or partially cooked mechanically tenderized beef product that contains added solution also declare the percentage of added solution?

Yes. However, there are different options for declaring the total amount of solution added. See 9 CFR 317.2(e)(2).

Q17. Do the new labeling requirements apply to raw or partially cooked beef products that have been marinated in a tumbler or vacuum tumbled?

The rule only applies to raw or partially cooked beef products that have been mechanically tenderized by needle or blade. This rule does not apply to other processes, such as tumbling or vacuum tumbling, unless the product is also mechanically tenderized by needle or blade.

needle-tenderize-beef

Everything’s big in Australia: 6 dead from ‘thunderstorm asthma’ in Melbourne

Rohan Smith of news.au.com reports doors swung wide open at homes around Melbourne on Monday afternoon in hopes a looming thunderstorm would bring with it a cool change.

thunderstorm-asthmaIt did, but it carried with it something else. The combination of warm weather, a high pollen count and stormy conditions produced what experts call “thunderstorm asthma,” an extremely rare and dangerous phenomenon that saw Victoria run out of ambulances for an entire hour.

Fairfax reported on Tuesday that two people are believed to have died while waiting for ambulances on Monday.

Those who suffered were not prepared. Most said they hadn’t experienced asthma since childhood. They all had one thing in common: Hay fever.

“I’ve never had asthma but do get hay fever, mainly itchy eyes and sneezes, but there were weirdly no other hay fever symptoms (on Monday),” Kate Craig, from Melbourne’s inner west, told news.com.au.

“It hit me all of a sudden about 7.30 last night, I felt like I couldn’t take a full breath and had an awful, chesty, hacking cough. I thought I must have just inhaled some spices while cooking — that’s what it felt like.”

Gary Nunn said he was in transit when he began to have trouble breathing.

“I’d had bad hay fever all day in Melbourne. In the early evening I got to Melbourne Airport and noticed a new symptom: I was struggling to breathe. This had never happened before,” he said.

Will fewer people barf? FSMA and audits, especially third party, still suck

Allen L Mozek, MPH, a Fellow of the International Food Protection Training Institute (IFPTI) and public health advocate for prevention of areca nut diseases, writes in this (as yet unpublished) letter to the editor of Food Safety magazine that the October/November 2016 issue sings “Kumbaya” about the Food Safety Moderization Act (FSMA)  – let’s step back and be politically incorrect:

barf-o-meter-dec-12HACCP was never supposed to be a regulation but “an approach to inspection” – per Dr. Frank L. Bryan – HACCP pioneer.

Ready-to-eat produce and sprouts, due to outbreaks and lack of CCP, would not be permitted if not for Salinas CA producers and sprout association lobbying.

Preventive controls is “another regulation” that will mitigate hazards but not provide the conventional 5 log reduction of pathogens with these products.

Second, food fraud is intentional adulteration for economic gain and not amenable to either HACCP or preventive controls but is rather an ethical problem. See the essay:

Market Economies With Churches and Market Economies Without Churches, by Zhao Xiao

Third, I would like to see thorough answers to three questions (courtesy of economist Thomas Sowell):

1.     What is the empirical evidence for this legislation?

2.     Compared to what will this legislation be an improvement?

3.     What is the cost?

Fourth, please answer the charge of “conflict of interest” and “sham”, regarding third party auditors, exclaimed by renowned foodborne illness attorney Bill Marler and barfblog.com editor Doug Powell.

Washing is futile: Leafy green cone of silence

Lettuce is overrated.

lettuce-skullThat’s my response to people who ask about the proportionally high rates of foodborne illness in lettuce and other leafy greens eaten raw.

I like spinach – in a lasagna or stir-fry – but not raw.

Raw is risky.

There’s a bunch of new findings on foodborne pathogens and leafy greens which are summarized below.

In the sphere of public conversation, it is notable the Leafy Greens Marketing Agreement, the group formed after the 2006 E. coli-in-spinach outbreak that killed four and sickened at least 200 in the U.S. – has been once again silent on any research or outbreaks that associate risk with greens.

The leafy green cone of silence.

Investigations by University of Leicester microbiologists have revealed that just a small amount of damage to salad leaves can massively stimulate the presence of the food Salmonella in ready-prepared salad leaves.  

The scientists have discovered that juices released from damaged leaves also had the effect of enhancing the virulence of the pathogen, potentially increasing its ability to cause infection in the consumer.  

The research is led by Dr Primrose Freestone of the University’s Department of Infection, Immunity and Inflammation and PhD student Giannis Koukkidis, who has been funded by a Biotechnology and Biological Sciences Research Council (BBSRC) i-case Studentship.  

Their research investigates novel methods of preventing food poisoning pathogens from attaching to the surface of salad leaves to help producers improve food safety for consumers.

This latest study, published in Applied and Environmental Microbiology, found that juices from damaged leaves in bagged spinach and mixed salad increased Salmonella pathogen growth 2400-fold over a control group and also enhanced their adherence to surfaces and overall virulence, or capacity to cause disease.  

Dr Freestone said: “Salad leaves are cut during harvesting and we found that even microliters of the juices (less than 1/200th of a teaspoon) which leach from the cut-ends of the leaves enabled Salmonella to grow in water, even when it was refrigerated. These juices also helped the Salmonella to attach itself to the salad leaves so strongly that vigorous washing could not remove the bacteria, and even enabled the pathogen to attach to the salad bag container.  

“This strongly emphasizes the need for salad leaf growers to maintain high food safety standards as even a few Salmonella cells in a salad bag at the time of purchase could become many thousands by the time a bag of salad leaves reaches its use by date, even if kept refrigerated. Even small traces of juices released from damaged leaves can make the pathogen grow better and become more able to cause disease.  

 “It also serves as a reminder to consume a bagged salad as soon as possible after it is opened. We found that once opened, the bacteria naturally present on the leaves also grew much faster even when kept cold in the fridge.  

“This research did not look for evidence of Salmonella in bagged salads. Instead, it examined how Salmonella grows on salad leaves when they are damaged.”  

Leafy green and other salad vegetables are an important part of a healthy diet, providing vitamins, minerals, and dietary fiber. Ready to eat prepared salads are particularly popular, are widely consumed and so of significant economic importance. Over recent years there has however been a number of outbreaks associated with fresh salad produce contaminated with Salmonella and E. coli both in the USA and Europe. 

lettuce-harvestThis has triggered considerable interest in effective strategies for controls and interventions measures both in UK industry, the EU and key research funding bodies. 

Despite a number of published reports on improving the microbiological safety of salad leaf production, very few studies have investigated the behavior of Salmonella once the leaves have been bagged.  

Giannis said: “Anything which enhances adherence of foodborne pathogens to leaf surfaces also increases their persistence and ability to resist removal, such as during salad washing procedures.  Even more worrying for those who might eat a Salmonella contaminated salad was the finding that proteins required for the virulence (capacity to cause infection) of the bacteria were increased when the Salmonella came into contact with the salad leaf juices. “Preventing enteric pathogen contamination of fresh salad produce would not only reassure consumers but will also benefit the economy due to fewer days lost through food poisoning. We are now working hard to find ways of preventing salad-based infections.”  

No comment from the LGMA.

While this research may make it seem like pre-packaged salads pose a scary risk, the researchers themselves were quick to say they still eat bagged salads. But they make sure to look for packages that have appropriate use-by dates and crisp-looking leaves. They stay away from salads that have mushy, slimy-looking greens, or bags with accumulated salad juice at the bottom. And they make sure to eat the greens within one day of purchase.

“Our project does not indicate any increased risk to eating leafy salads, but it does provide a better understanding of the factors contributing to food poisoning risks,” said Freestone. 

If you feel like it, you can wash greens that have already been pre-washed by manufacturers just before eating, but Freestone says this doesn’t have much of an effect on the salmonella bacteria that may already be attached or internalized by the leaves. 

Her study was published in the journal Applied and Environmental Microbiology.

No comment from LGMA.

Assessment of irrigation water quality and microbiological safety of leafy greens in different production systems

Journal of Food Safety, 2 November 2016, DOI: 10.1111/jfs.12324

http://onlinelibrary.wiley.com/doi/10.1111/jfs.12324/abstract;jsessionid=883317B2001984CC39815B1792B68759.f04t01

Foodborne disease outbreaks associated with fresh produce irrigated with contaminated water are a constant threat to consumer health. In this study, the impact of irrigation water on product safety from different food production systems (commercial to small-scale faming and homestead gardens) was assessed.

spongebob-oil-colbert-may3-10Hygiene indicators (total coliforms, Escherichia coli), and selected foodborne pathogens (Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7) of water and leafy green vegetables were analyzed. Microbiological parameters of all irrigation water (except borehole) exceeded maximum limits set by the Department of Water Affairs for safe irrigation water. Microbial parameters for leafy greens ranged from 2.94 to 4.31 log CFU/g (aerobic plate counts) and 1 to 5.27 log MPN/100g (total coliforms and E. coli). Salmonella and E. coli O157:H7 were not detected in all samples tested but L. monocytogenes was present in irrigation water (commercial and small-scale farm, and homestead gardens).

This study highlights the potential riskiness of using polluted water for crop production in different agricultural settings.

No comment from LGMA.

Adaptive response of Listeria monocytogenes to heat, salinity and low pH, after habituation on cherry tomatoes and lettuce leaves

PLOS

Sofia V. Poimenidou, Danai-Natalia Chatzithoma, George-John Nychas, Panagiotis N. Skandamis

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165746

Pathogens found on fresh produce may encounter low temperatures, high acidity and limited nutrient availability. The aim of this study was to evaluate the effect of habituation of Listeria monocytogenes on cherry tomatoes or lettuce leaves on its subsequent response to inhibitory levels of acid, osmotic and heat stress.

Habituation was performed by inoculating lettuce coupons, whole cherry tomatoes or tryptic soy broth (TSB) with a three-strains composite of L. monocytogenes, which were further incubated at 5°C for 24 hours or 5 days. Additionally, cells grown overnight in TSB supplemented with 0.6% yeast extract (TSBYE) at 30°C were used as control cells. Following habituation, L. monocytogenes cells were harvested and exposed to: (i) pH 3.5 adjusted with lactic acid, acetic acid or hydrochloric acid (HCl), and pH 1.5 (HCl) for 6 h; (ii) 20% NaCl and (iii) 60°C for 150 s.

Results showed that tomato-habituated L. monocytogenes cells were more tolerant (P < 0.05) to acid or osmotic stress than those habituated on lettuce, and habituation on both foods resulted in more stress resistant cells than prior growth in TSB. On the contrary, the highest resistance to heat stress (P < 0.05) was exhibited by the lettuce-habituated L. monocytogenes cells followed by TSB-grown cells at 5°C for 24 h, whereas tomato-habituated cells were highly sensitized. Prolonged starvation on fresh produce (5 days vs. 24 h) increased resistance to osmotic and acid stress, but reduced thermotolerance, regardless of the pre-exposure environment (i.e., tomatoes, lettuce or TSB).

lettuce-woolies-sep_-12-300x225These results indicate that L. monocytogenes cells habituated on fresh produce at low temperatures might acquire resistance to subsequent antimicrobial treatments raising important food safety implications.

No comment from LGMA.

Efficacy of post-harvest rinsing and bleach disinfection of E. coli O157:H7 on spinach leaf surfaces

Food Microbiology, April 2017, vol 62, pg 212-220, http://dx.doi.org/10.1016/j.fm.2016.10.019

Attachment and detachment kinetics of Escherichia coli O157:H7 from baby spinach leaf epicuticle layers were investigated using a parallel plate flow chamber. Mass transfer rate coefficients were used to determine the impact of water chemistry and common bleach disinfection rinses on the removal and inactivation of the pathogen. Attachment mass transfer rate coefficients generally increased with ionic strength. Detachment mass transfer rate coefficients were nearly the same in KCl and AGW rinses; however, the detachment phase lasted longer in KCl than AGW (18 ± 4 min and 4 ± 2 min, respectively), indicating that the ions present during attachment play a significant role in the cells’ ability to remain attached. Specifically, increasing bleach rinse concentration by two orders of magnitude was found to increase the detachment mass transfer rate coefficient by 20 times (from 5.7 ± 0.7 × 10−11 m/s to 112.1 ± 26.8 × 10−11 m/s for 10 ppb and 1000 ppb, respectively), and up to 88 ± 4% of attached cells remained alive.

lettuceThe spinach leaf texture was incorporated within a COMSOL model of disinfectant concentration gradients, which revealed nearly 15% of the leaf surface is exposed to almost 1000 times lower concentration than the bulk rinse solution.

No comment from LGMA.

Development of growth and survival models for Salmonella and Listeria monocytogenes during non-isothermal time-temperature profiles in leafy greens

Food Control, Vol 71, Part A, Jan 2017, Pg 32-41

http://dx.doi.org/10.1016/j.foodcont.2016.06.009

http://www.sciencedirect.com/science/article/pii/S0956713516303164

Leafy greens contaminated with Salmonella enterica have been linked to large number of illnesses in many countries in recent years. Listeria monocytogenes is also a pathogen of concern for leafy greens because of its prevalence in the growing and processing environment and its ability to grow at refrigeration temperatures. Experimental data for the growth and survival of S. enterica and L. monocytogenes under different conditions and storage temperatures were retrieved from published studies. Predictive models were developed using the three-phase linear model as a primary growth model and square-root model to calculate specific growth rate (ln CFU g−1 h−1) at different temperatures (°C). The square-root model for S. enterica was calculated as μ = (0.020(Temperature+0.57))2. The square-root model for L. monocytogenes was fitted as μ = (0.023(Temperature-0.60))2. The growth-survival model for S. enterica and growth model for L. monocytogenes were validated using several dynamic time-temperature profiles during the production and supply chain of leafy greens. The models from this study will be useful for future microbial risk assessments and predictions of behavior of S. enterica and L. monocytogenes in the leafy greens production and supply chain.

No comment from LGMA.

 

Is there a relation between the microscopic leaf morphology and the association of Salmonella and Escherichia coli O157:H7 with iceberg lettuce leaves?

Journal of Food Protection, Number 10, October 2016, pp. 1656-1662, pp. 1784-1788(5)

I Van der Linden, M  Eriksson, M Uyttendaele, F Devlieghere

http://www.ingentaconnect.com/content/iafp/jfp/2016/00000079/00000010/art00017

To prevent contamination of fresh produce with enteric pathogens, more insight into mechanisms that may influence the association of these pathogens with fresh produce is needed.

lettuce-washIn this study, Escherichia coli O157:H7 and Salmonella were chosen as model pathogens, and fresh cut iceberg lettuce was chosen as a model fresh produce type. The morphological structure of iceberg lettuce leaves (stomatal density and length of cell margins per leaf area) was quantified by means of leaf peels and light microscopy of leaves at different stages of development (outer, middle, and inner leaves of the crop) on both leaf sides (abaxial and adxial) and in three leaf regions (top, center, and bottom). The morphology of the top region of the leaves was distinctly different from that of the center and base, with a significantly higher stomatal density (up to five times more stomata), different cell shape, and longer cell margins (two to three times longer). Morphological differences between the same regions of the leaves at different stages of development were smaller or nonsignificant. An attachment assay with two attenuated E. coli O157:H7 strains (84-24h11-GFP and BRMSID 188 GFP) and two Salmonella strains (serovars Thompson and Typhimurium) was performed on different regions of the middle leaves. Our results confirmed earlier reports that these pathogens have a higher affinity for the base of the lettuce leaf than the top. Differences of up to 2.12 log CFU/g were seen (E. coli O157:H7 86-24h11GFP). Intermediate attachment occurred in the central region.

The higher incidence of preferential bacterial attachment sites such as stomata and cell margins or grooves could not explain the differences observed in the association of the tested pathogens with different regions of iceberg lettuce leaves.

No comment from LGMA.

The N.Y Times reports the one place the one place the Salinas Valley’s bounty of antioxidants does not often appear is on the tables of the migrant workers who harvest it.

sponge-bob-handwashingMore than a third of the children in the Salinas City Elementary School District are homeless; overall diabetes rates are rising and projected to soar; and 85 percent of farmworkers in the valley are overweight or obese, partly because unhealthy food is less costly, said Marc B. Schenker, a professor at the University of California, Davis, who studies the health of farmworkers.

No comment from LGMA.

Sponge Bob wants answers.

Fancy food ain’t safe food Denmark edition: Country’s only three-star restaurant fined £2,300 for hygiene breaches

Denmark’s only three-star Michelin restaurant on Thursday faced questions over hygiene after it was fined 20,000 kroner (£2,300) by the country’s food safety authority.

restaurant-geranium-iiGeranium, the first eatery in Denmark to receive top Michelin honours, had been storing fresh shellfish such as oysters, crayfish and scallops in temperatures that were too warm and over an extended period, the Danish Food Administration wrote after an inspection.

Two walk-in coolers also had “black, green and white splotches growing on the underside of shelves and on packaged pickled garlic”, according to a report dated 29 September but picked up by Danish media only on Thursday.

The regulator awarded the Copenhagen restaurant – which charges 2,000 kroner for a meal without drinks – a frowning “smiley,” the lowest grade of its four-tier system.

Geranium chef Rasmus Kofoed told Danish news agency Ritzau: “I do not agree with what is written. I believe that it is greatly exaggerated but I admit that there are some parts of the process where perhaps we have been a bit unattentive.”

geranium-denmarkLess talk, more action.

The restaurant had been using a computerized system to monitor food temperatures incorrectly, but fish and shellfish were always stored on ice regardless of the surrounding temperature, he added.

This year the Nordic edition of the Michelin Guide gave three stars to Geranium, but only two to Copenhagen’s celebrated Noma, which was named best restaurant in the world by Britain’s Restaurant magazine in 2010, 2011, 2012 and 2014.

Noma too faced criticism from the Danish food safety regulator in 2013, when it was accused of not taking adequate action after a sick kitchen worker gave dozens of customers food poisoning.

The gift that keeps on giving.

smiley-faces-denmark-rest-inspection

Brisbane-area vehicles covered in human poop

Clare Armstrong of The Courier Mail reports that two vehicles ended up covered in human waste after a truck carrying 27 tonnes of sewage was forced to brake suddenly to avoid a collision at Toowoomba.

poop-spill-brisbaneIt is understood the truck transporting the sewage lost its load when the driver braked suddenly to avoid crashing into a car outside The Big Orange fruit barn on Crowley Vale Road on Wednesday just after 8am.

The Arkwood Organic Recycling (is there any other kind of recycling shit other than organic?) truck trailer had a tarpaulin-like cover over the top but the force of the braking action caused it to rip apart and the waste spilt out.

Shocked locals took to social media to express their disgust at the scene as the truck, car and surrounding area were covered in human waste.

oddball-kellys-heros“Where’s the gag reflex,” posted one person.

“You can still get through it,” advised another person online.

Arkwood Organic Recycling Owner and Operations Manager Brendon Clarke told local media the load was secured but the abrupt stop caused the trailer’s cover on the truck to tear.