An elderly woman who was admitted to Sunderland Royal Hospital after eight cases of Samonella were confirmed at Millum House Care Home in Roker, Sunderland, has died.
A post-mortem examination to establish the cause of her death is to be carried out.
The news follows the death of great-grandmother Myra Robinson, 72, who died in hospital last Saturday following the outbreak.
The remaining patients, who include three members of staff at the three-storey home, have recovered.
Health chiefs are investigating, but the cause of the outbreak still remains unclear.