Building models to predict and prevent Salmonella outbreaks

Researchers at the University of Minnesota School of Public Health (SPH) and College of Veterinary Medicine are working together with industry to determine how high Salmonella levels in ground turkey and ground beef have to be before they pose a threat to human health.

ground.turkey“Ultimately we are trying to better understand how to predict and prevent outbreaks,” says Craig Hedberg, SPH professor and one of the researchers working on the study, Developing a Risk Management Framework to Improve Public Health Outcomes by Enumerating Salmonella in Ground Meat and Poultry Products.

“It would be easy to say we should have no Salmonella in [meat] products, but that carries risks, too,” says Hedberg. “Meat with contamination levels too low to cause illness in people would be destroyed, and [that could lead] the industry to stop or reduce its current level of testing, which would halt the monitoring process and increase the risk of illness.”

To find a workable solution to the issue, the research team will use data from Cargill and other industry partners as well as data on past outbreaks to compare Salmonella concentrations under normal production conditions in ground meat products with levels that have been linked to the occurrence of foodborne illness outbreaks.

The team is focusing on ground meat because grinding mixes contamination throughout the product, and live bacteria can remain inside an undercooked hamburger or turkey burger.

Theatre or threat? Food safety concerns at Brazil’s Olympic stadium

Authorities have seized more than 45 kg of expired food during a raid of the Maracana Stadium, the showpiece venue for next year’s Olympics.

maracanaAgents from local consumer protection watchdog Procon found the products during Sunday’s football derby between Fluminense and Botafogo, Procon said in a statement on Monday. Among the items confiscated were sausages, eggs, pork, chocolate and cheese, reports Xinhua.

The Maracana Stadium hosted last year’s FIFA World Cup final between Germany and Argentina after undergoing a three-year, $500 million upgrade. The venue will also stage the opening and closing ceremonies, and football matches, at next year’s Olympics.

 

Hong Kong bans ‘horse penis’ clams: Canadian industry in peril

They can weigh more than 5kg, live more than 150 years and by common consensus resemble a certain part of a male horse’s anatomy.

3-diver_showing_geoduck_under_waterThe geoduck clam is also delicious and represents a C$50 million (HK$307 million) annual export industry for British Columbia.

That industry is now hanging in the balance, after Hong Kong banned all geoduck imports from the Canadian province on December 24, upon the detection of Paralytic Shellfish Poisoning (PSP) toxin in a sample of BC clams.

Hong Kong previously imported more than 70 per cent of BC’s entire geoduck harvest of 1.45 million kg per year. Most of that was shipped onwards to mainland China and elsewhere, but the ban covers all SAR imports, regardless of whether they were destined for local diners.

Not just from watching the show: Australian vet barfs on I’m a Celebrity … Get Me Out Of Here

It’s usually the celebs that end up vomiting on I’m A Celebrity … Get Me Out Of Here!

celebrity.barf.aust.mar.15But this time, it was Dr Chris Brown that couldn’t keep his food down.

Freddie Flintoff had the task of chowing down on critters during Tuesday night’s tucker trial.

He managed to down a giant snail, a cockroach and a rats tail but had to bow out when served a “critters jungle juice” — a smoothie of cockroaches, worms, fly pupae and crickets.

That’s when Dr Chris Brown decided to step in and see for himself how bad it was.

And it wasn’t good.

Dr Chris had to walk away to vomit into the bushes — leaving his co-host, Julia Morris, to manage the rest of the challenge.

That’s when Freddie stepped in to offer a rather robotic impression of the Bondi Vet.

Roadkill is his only red meat

In its continuing quest for food porn, NPR quotes Jeff Potter as saying, “Last autumn, my brother phones on his way home from the grocery: ‘I was driving to the store and there wasn’t a deer in the road, but on the way back there was, so it’s gotta be fresh!’ “

roadkill.deerPotter, who lives in exurban Lansing, Mich., was busy processing mail orders for his outdoor sports business, but he knew he had to act fast or someone might beat him to it. He spread a tarp in the back of the family minivan and raced to the scene, where he found a young doe on the shoulder of the road. He pulled the deer into the van, then called the police for permission to take it home. To eat.

Potter is a 53-year-old father of two who operates Out Your Backdoor, a website dedicated to “indie” outdoor culture. He has hunted, fished, biked and skied around Williamston, Mich., his whole life. Today it’s much less rural than it was when he was a kid, but “there’s still this tremendous amount of interstitial space that deer thrive in,” he says. And during the fall mating season, when the animals start getting frisky, “there are tremendous numbers of car-deer accidents around here.”

Potter is known among his friends and family for collecting roadkill of all species: deer, pheasant, turkey, rabbit, squirrel. They call him when they spot felled critters by the roadside, and he serves stews and roasts made from them at family dinners and large dinner parties. How do his guests rate the meals? So far, he says, he’s “batting 1,000.” Roadkill venison makes up the near totality of the red meat his family consumes. And no one’s ever gotten sick.

Not that he knows of.

E. coli in Australian pigs

Background: Enterotoxigenic Escherichia coli (ETEC) are a major economic threat to pig production globally, with serogroups O8, O9, O45, O101, O138, O139, O141, O149 and O157 implicated as the leading diarrhoeal pathogens affecting pigs below four weeks of age.

kid_pig_kissA multiple antimicrobial resistant ETEC O157 (O157 SvETEC) representative of O157 isolates from a pig farm in New South Wales, Australia that experienced repeated bouts of pre- and post-weaning diarrhoea resulting in multiple fatalities was characterized here. Enterohaemorrhagic E. coli (EHEC) O157:H7 cause both sporadic and widespread outbreaks of foodborne disease, predominantly have a ruminant origin and belong to the ST11 clonal complex. Here, for the first time, we conducted comparative genomic analyses of two epidemiologically-unrelated porcine, disease-causing ETEC O157; E. coli O157 SvETEC and E. coli O157:K88 734/3, and examined their phylogenetic relationship with EHEC O157:H7.

Results: O157 SvETEC and O157:K88 734/3 belong to a novel sequence type (ST4245) that comprises part of the ST23 complex and are genetically distinct from EHEC O157. Comparative phylogenetic analysis using PhyloSift shows that E. coli O157 SvETEC and E. coli O157:K88 734/3 group into a single clade and are most similar to the extraintestinal avian pathogenic Escherichia coli (APEC) isolate O78 that clusters within the ST23 complex. Genome content was highly similar between E. coli O157 SvETEC, O157:K88 734/3 and APEC O78, with variability predominantly limited to laterally acquired elements, including prophages, plasmids and antimicrobial resistance gene loci. Putative ETEC virulence factors, including the toxins STb and LT and the K88 (F4) adhesin, were conserved between O157 SvETEC and O157:K88 734/3. The O157 SvETEC isolate also encoded the heat stable enterotoxin STa and a second allele of STb, whilst a prophage within O157:K88 734/3 encoded the serum survival gene bor. Both isolates harbor a large repertoire of antibiotic resistance genes but their association with mobile elements remains undetermined.

flying.pig.kids.in.the.hallConclusions: We present an analysis of the first draft genome sequences of two epidemiologically-unrelated, pathogenic ETEC O157. E. coli O157 SvETEC and E. coli O157:K88 734/3 belong to the ST23 complex and are phylogenetically distinct to EHEC O157 lineages that reside within the ST11 complex.

Comparative genomic analysis of a multiple antimicrobial resistant enterotoxigenic E. coli O157 lineage from Australian pigs

BMC Genomics 2015, 16:165

Ethan Wyrsch, Piklu Roy Chowdhury, Sam Abraham, Jerran Santos, Aaron E Darling, Ian G Charles, Toni A Chapman and Steven P Djordjevic

http://www.biomedcentral.com/1471-2164/16/165/abstract

Possum pesticide: Blackmailers threaten to poison New Zealand infant milk formula

A blackmail threat to poison baby formula is a form of “eco-terrorism” says New Zealand’s prime minister John Key.

possum.baby.nov.11New Zealand police are currently investigating blackmail threats made against dairy processor Fonterra.

The threats were made as part of a campaign to stop the use of agricultural pesticide 1080 in New Zealand.

New Zealand police said that anonymous letters were received by Federated Farmers and Fonterra in November 2014, accompanied by small packages of milk powder, which subsequently tested positive for the presence of a concentrated form of 1080.

The letters threatened to contaminate infant and other formula with 1080 (sodium monoflouroacetate) unless New Zealand stopped using it for pest control by the end of March 2015.

“We have tested just over 40,000 raw milk and product samples and we have had no 1080 detections,” said a statement from the NZ Ministry for Primary Industries.

Asked how he would characterise the threat, Mr Key replied: “It’s a form of eco-terrorism without doubt.

Authorities warned parents to examine packaging for signs of tampering and supermarkets removed formula cans from shelves to storerooms so shoppers could not access them directly.

baby_formulaDeputy Commissioner of National Operations with NZ Police, Mike Clement, said the threat may be a hoax, but must be treated seriously.

He said no further letters had been received after the initial batch and the matter was being treated as blackmail rather than terrorism.

President of New Zealand’s peak farming body, Federated Farmers of New Zealand (FFNZ), William Rolleston, said he was confident security measures at the country’s milk processing plants were rigorous.

“I would say that it would probably be easier to break out of prison, than to break into one of these factories and do anything, the security is pretty tight.” Dr Rolleston said.

He said he believed his organisation was targeted by the anonymous letter writer due to their support for the use of 1080 to control possum populations.

“Unlike you guys in Australia who protect your possums, they are not a welcome visitor here I’m afraid.

“We do endorse the use of 1080, it’s a highly effective and safe product when used properly and it’s biodegradable, so it breaks down in the environment very quickly, it doesn’t have an impact on our native species, so it’s a very good toxin to use for dealing with what is a major issue for New Zealand.”

The New Zealand government has additional information at http://www.foodprotection.govt.nz/

E. coli O26 found in French cheese Roquefort

Escherichia coli O26 has been discovered in Roquefort cheese, reports the Netherlands Food and Consumer Product Safety Authority (NVWA), based on information received from the Rapid Alert System for Food and Feed (RASFF).

Roquefort_cheeseThe Roquefort is a raw milk cheese with barcode EE1632, identification FR 120203 022CE. It was sold in supermarkets in 100gm units with the expiration date May 8th, 2015. It has been advised not to consume the cheese and if already purchased, consumers need to throw it away immediately.

Cooking E. coli out of veal cordon bleu

Veal cutlets were surface inoculated with ca. 6.6 cfu/g of an eight-strain rifampicin-resistant cocktail of Shiga toxin-producing Escherichia coli (STEC) (O26:H11, O45:H2, O103:H2, O104:H4, O111:H-, O121:H19, O145:NM and O157:H7). Cutlets were mechanically tenderized and cordon bleu was prepared by adding slices of ham and cheese between two cutlets prior to batter/breading and cooking.

Veal_Cordon_Bleu_-_1024-512x288Fully assembled cordon bleu were cooked in preheated (191.5C) extra virgin olive oil (45 mL) on a griddle. Cooking for 4, 5 or 6 min per side reduced STEC levels by ca. 1.3, 2.2 or 3.4 log cfu/g, respectively, whereas cooking for 7–10 min per side resulted in reductions of ca. ≥6.2 log cfu/g.

These data validated that cooking tenderized veal cordon bleu for at least 7 min per side in 45 mL of olive oil on a griddle maintained at ca. 191.5C is sufficient to achieve an internal cordon bleu temperature of 69.0 ± 3.3C and a ≥5-log reduction of STEC.

Thermal inactivation of Shiga toxin-producing Escherichia coli cells within veal cordon bleu

Journal of Food Safety [ahead of print]

Kulas, M., Porto-Fett, A. C.S., Swartz, R. S., Shane, L. E., Strasser, H., Munson, M., Shoyer, B. A. and Luchansky, J. B.

http://onlinelibrary.wiley.com/doi/10.1111/jfs.12188/abstract;jsessionid=FCEB8A5A4EB2E460A51BEEC27D419A8B.f03t03

Pigeon, parakeet and pony: Amsterdam food truck serves maligned meat

I don’t care what various cultures use as their source of protein: I care that it is microbiologically safe.

cackling-geese1According to NPR, in Amsterdam, two artists are trying to widen their city’s list of local tasty creatures — and expand minds, too — with dishes like the My Little Pony Burger, Peace Pigeon and Bambi Ball.

Their project, The Kitchen Of The Unwanted Animal is a food truck and specialty food provider featuring animals that are, generally, considered pests and almost always considered inedible.

“I think there is a kind of block in your head because it’s a pet or [an animal that’s not typically eaten],” says Rob Hagenouw, 55, one of the founders of the Kitchen. “Here we have pet, pest and eating animals — and we don’t mix them.” But he and his partner, Nicolle Schatborn, 51, are trying to show their neighbors that these animals can be delicious, and shouldn’t be wasted.

wholetruck-3aaab70a52e0d7f294c62882b25772c5fd90da71-s1600-c85It all started five years ago with a wild goose stew Schatborn and Hagenouw made for an art fair as part of a larger installation. The stew got them wondering about what happened to geese and other animals that were considered “unwanted” in Holland.

I know a lot of North Americans, especially on golf courses, that would want to get rid of the geese.