Surely you can’t be cereus: Pay attention to temperature control, esp when cooking for large numbers

The aim of this study was to identify and characterise Bacillus cereus from a unique national collection of 564 strains associated with 140 strong-evidence food-borne outbreaks (FBOs) occurring in France during 2007 to 2014.

surely-you-cant-be-seriousStarchy food and vegetables were the most frequent food vehicles identified; 747 of 911 human cases occurred in institutional catering contexts. Incubation period was significantly shorter for emetic strains compared with diarrhoeal strains.

A sub-panel of 149 strains strictly associated to 74 FBOs and selected on Coliphage M13-PCR pattern, was studied for detection of the genes encoding cereulide, diarrhoeic toxins (Nhe, Hbl, CytK1 and CytK2) and haemolysin (HlyII), as well as panC phylogenetic classification. This clustered the strains into 12 genetic signatures (GSs) highlighting the virulence potential of each strain. GS1 (nhe genes only) and GS2 (nhe, hbl and cytK2), were the most prevalent GS and may have a large impact on human health as they were present in 28% and 31% of FBOs, respectively.

Our study provides a convenient molecular scheme for characterisation of B. cereus strains responsible for FBOs in order to improve the monitoring and investigation of B. cereus-induced FBOs, assess emerging clusters and diversity of strains.

Baccillus cereus-induced food-borne outbreaks in France, 2007 to 2014: Epidemiology and genetic characteristics

Eurosurveillance, Vol 21, Issue 48, 01 December 2016

http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=22657

‘Dead man’s tray’ Clostridium perfringens likely cause of Fargo jail outbreak

Three weeks after an apparent foodborne illness outbreak at the Cass County Jail, the North Dakota Department of Health determined what likely made about 110 inmates sick over a two-day stretch in mid-December.

fargo.margAlthough an official cause is still pending, State Epidemiologist Laura Cronquist says testing by the U.S. Centers for Disease Control and Prevention points to Clostridium perfringens bacteria as the culprit, based on stool samples from several inmates.

Clostridium perfringens is commonly found on raw meat and poultry, according to the CDC, and infection can occur when foods are prepared in large quantities and kept warm for a long time before serving.

“It’s really common,” Cronquist said, adding, “It’s not really surprising that it’s an institution that this happened in.”

Last month’s outbreak was the second major outbreak at the Cass County Jail in four years. In the latest case, about 40 percent of the jail’s 282 inmates had symptoms including diarrhea and nausea. No inmates were hospitalized, and jail staff said the illness was short-lived.

Clostridium perfringens was also the likely cause of a larger illness outbreak at the jail in November 2011, when 90 percent of the 184 inmates inmates came down with diarrhea and vomiting. In that case, the organism was also found inside sick inmates, but couldn’t be confirmed in the most likely food source, the chili macaroni served that day.

The Cass County Jail freezes meal samples daily to save in the event of illness in a process referred to in the corrections industry as a “dead man’s tray.” Cronquist said she’s working with the CDC to determine which of those specific foods will be tested.

Buffett from hell: 31 sickened with Staph at a horsey event in Luxembourg

In June 2014, a staphylococcal food poisoning outbreak occurred at an international equine sports event in Luxembourg requiring the hospitalization of 31 persons.

horseWe conducted a microbiological investigation of patients and buffet items, a case–control study and a carriage study of catering staff. Isolates of Staphylococcus aureus from patients, food and catering staff were characterized and compared using traditional typing methods and whole genome sequencing.

Identical strains (sequence type ST8, spa-type t024, MLVA-type 4698, enterotoxin A FRI100) were isolated in 10 patients, shiitake mushrooms, cured ham, and in three members of staff. The case–control study strongly suggested pasta salad with pesto as the vehicle of infection (p<0.001), but this food item could not be tested, because there were no leftovers. Additional enterotoxigenic strains genetically unrelated to the outbreak strain were found in four members of staff. Non-enterotoxigenic strains with livestock-associated sequence type ST398 were isolated from three food items and two members of staff.

The main cause of the outbreak is likely to have been not maintaining the cold chain after food preparation. Whole genome sequencing resulted in phylogenetic clustering which concurred with traditional typing while simultaneously characterizing virulence and resistance traits.

 Investigation of a Staphylococcal food poisoning outbreak combining case control, traditional typing, and whole genome sequencing methods

Eurosurveillance, Volume 20, Issue 45, November 2015

  1. Mossong, F. Decruyenaere, G. Moris, C. Ragimbeau, C.M. Olinger, S. Johler, M. Perrin, P. Hau, P. Weicherding

http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=21301